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<asp:GridView ID="GridView1" runat="server" BorderColor="#666666" 
  BorderStyle="Solid" BorderWidth="1px" OnRowDataBound="GridView1_RowDataBound">
  <HeaderStyle BackColor="#999999" Font-Bold="False" Font-Overline="False" 
   Font-Underline="False" ForeColor="Black" HorizontalAlign="Center" />
  <AlternatingRowStyle BackColor="#E8E8E8" />
</asp:GridView>

protected void GridView1_RowDataBound(object sender, GridViewRowEventArgs e)
{
e.Row.Cells[0].Visible = false;
}